In this tutorial we will use the Custom genotyping plugin to screen whole genome sequences of Salmonella for acquired and mutational resistance.
PDF file:
Download sample data:
Custom knowledgebases
Knowledge bases for use with the Custom genotyping plugin.
Download demonstration database:
WGS_demo_database_for_Salmonella_enterica
Demonstration database containing data for a set of 62 Salmonella enterica isolates. This database uses publicly available next-generation sequence reads from the Sequence Read Archive (SRA). For each isolate, NGS reads were de novo assembled into genome sequences. wgMLST alleles were called using the assembly-based and assembly-free method.
Note that the downloaded database backup file (.bnbk) can be restored via the Restore database... functionality in the BIONUMERICS startup screen.