In this tutorial we will screen whole genome sequences of any Gram-negative bacterial samples for phenotypic antibiotic resistance traits using the Resistance detection plugin.
PDF file:
Download demonstration database:
WGS_demo_database_for_Escherichia_coli
Demonstration database containing data for a set of 60 Escherichia coli isolates. This database uses publicly available next-generation sequence reads from the Sequence Read Archive (SRA). For each isolate, NGS reads were de novo assembled into genome sequences. wgMLST alleles were called using the assembly-based and assembly-free method.
Note that the downloaded database backup file (.bnbk) can be restored via the Restore database... functionality in the BIONUMERICS startup screen.