A tutorial for working with the whole genome Multi Locus Sequence Typing (wgMLST) demonstration database of Staphylococcus aureus, which allows BioNumerics users to explore the wgMLST functionality without the need for a wgMLST project or Calculation Engine credits.
wgMLST analysisWhole-genome Multi-Locus Sequence Typing (wgMLST) using BIONUMERICS. See the wgMLST application page for a complete overview.
Demonstration database containing data for a set of 97 Staphylococcus aureus isolates originating from three published studies. This database uses publicly available next-generation sequence reads from the Sequence Read Archive (SRA). For each isolate, NGS reads were de novo assembled into genome sequences. wgMLST alleles were called using the assembly-based and assembly-free method.
Note that the downloaded database backup file (.bnbk) can be restored via the Restore database... functionality in the BIONUMERICS startup screen.