Follow-up analysis of MLST data

In this tutorial you will learn how to perform some cluster analyses (UPGMA tree, minimum spanning tree, maximum parsimony tree, ....) on MLST data. You will also learn how to alter the layout of the clusterings and how to export the pictures to use it in a publication, presentation, etc.

MLST analysis

Multi-Locus Sequence Typing (MLST) using the BIONUMERICS MLST online plugin. See the MLST application page for a complete overview.
Download PDF file: 
Download demonstration database: 
Neisseria MLST demo database

Demonstration database containing 500 Neisseria strains with sequences and allele IDs for the seven housekeeping genes, present the publicly available MLST scheme hosted at

Note that the downloaded database backup file (.bnbk) can be restored via the Restore database... functionality in the BIONUMERICS startup screen.