The Fragment Analyzer is a capillary electrophoresis instrument from the company Advanced Analytical Technologies (http://www.aati-us.com). The generated electropherograms can be exported from the AATI PROSize® data analysis software in csv or txt format and imported in BioNumerics after installation of the Import fingerprint tools plugin. In this tutorial the installation of the Import fingerprint tools plugin and the import and processing of the AATI Fragment Analyzer curve files is covered.
Any type of data that can be translated into a densitometric curve is considered a fingerprint type in the BIONUMERICS and GelCompar II software. This includes commonly used genotyping methods employing agarose or polyacrylamide slab gel electrophoresis (PFGE, rep-PCR, RAPD, PCR-DGGE, etc.), in which case the data are usually imported as two-dimensional gel images (bitmaps). Another major group consists of capillary electrophoresis profiles such as AFLP, ARISA, T-RFLP, etc. Here, the raw electropherograms generated by an automated sequencer (genetic analyzer) or derived peak table text files can be imported. Finally, any other profile (generated e.g. by gas chromatography, HPLC or spectrophotometry) that can be seen as peaks or bands, can be analyzed as a fingerprint.