In this tutorial we will search for the sequences corresponding to the seven housekeeping genes used in the online MLST scheme of Listeria monocytogenes. The assembled sequences present in the whole genome demonstration database of Listeria monocytogenes are used in this tutorial.
MLST analysis
Multi-Locus Sequence Typing (MLST) using the BIONUMERICS MLST online plugin. See the MLST application page for a complete overview.
Demonstration database containing data for a set of 51 Listeria monocytogenes isolates. This database uses publicly available next-generation sequence reads from the Sequence Read Archive (SRA). For each isolate, NGS reads were de novo assembled into genome sequences. wgMLST alleles were called using the assembly-based and assembly-free method.
Note that the downloaded database backup file (.bnbk) can be restored via the Restore database... functionality in the BIONUMERICS startup screen.