This tutorial illustrates the steps needed to set up your own MLST schema, e.g. in case you are studying a less common organism for which no MLST data are available yet.
MLST analysisMulti-Locus Sequence Typing (MLST) using the BIONUMERICS MLST online plugin. See the MLST application page for a complete overview.
Download PDF file:
Download sample data:
MLST sample AB1 trace files
A set of raw sequence trace files in .ab1 format for seven fictitious loci (wtlF, frt, mslS, orvL, mnkP, beeR, and wffL) used in the MLST scheme of a fictitious organism Ambiorix belgiensis.