During this three sessions webinar Typing with WGS in BIONUMERICS participants will get a good overview of all the possibilities of the BIONUMERICS software package for typing of bacterial isolates with Whole Genome Sequencing (WGS) through alternating theoretical sessions and hands-on practice sessions in the software. By the end of the workshop you should have clear insights into the main BIONUMERICS WGS applications, including:

  • The import of fastq files as sequence read sets, including data validation and cleaning
  • The import of genomes and metadata from public repositories or other sources
  • Mapping of reads against a reference genome
  • De novo assembly
  • Annotation of an assembled genome
  • Genotyping tools that allow to screen the strain resistome and virulome, and in addition allow determination of e.g. pathotype (E. coli), serotype (E. coli), spa type (S. aureus)…
  • Whole genome MLST (wgMLST) analysis, i.e. gene-by-gene systems as an extension of traditional 7-gene MLST
  • Whole genome SNP (wgSNP) analysis, i.e. the detection of SNPs on whole genomes sequences mapped against a reference sequence
  • Cluster analysis based on wgMLST and/or wgSNP

Practical information

The Typing with WGS webinar consists of three half days and will begin on Monday afternoon until Wednesday afternoon every day from 1.30 pm until 5 pm CET . There are no formal breaks during the webinar, but the time for the exercises is sufficient to allow participants to step away from the computer briefly. Webinar Notes are available free of charge and include hand-outs of all the lectures and tutorials of the hands-on exercises.

All participants are required to have BIONUMERICS installed on the PC they will use to connect to the webinar. Upon registration, you will receive a link to download the workshop notes and data used during the tutorials and you will be provided with detailed instructions to prepare your PC before the Webinar. A basic knowledge of the applications covered is presumed.

The number of participants to the webinar is limited to 20. In case the webinar is fully booked, the date of registration together with prepayment will be decisive. Applied Maths NV reserves the right to cancel this webinar up to one month before the scheduled date in case of insufficient registrations.

Registration fee

The registration for the three sessions webinar is 995 Euro (excl. VAT*).

(*) For Belgian customers 21% VAT will be applied. For other customers within the EU please provide the VAT registration number of your organization to be exempted from paying the VAT. Federal and local taxes may apply for non-EU customers.

Date: May 3rd to 5th, 2021

Location: online

Fully booked!