This tutorial illustrates how to import FASTQ file links into a BioNumerics database and finally how to perform a de novo assembly on the external calculation engine.
Sequence read sets
A sequence read set is designed to hold large sets of short reads generated by next generation sequencing (NGS). Base sequences and their associated quality scores are stored for single-end and paired-end reads, originating from various high-throughput sequencing platforms such as Illumina, Ion Torrent, PacBio, Oxford Nanopore, etc.
Demonstration database containing data for a set of 97 Staphylococcus aureus isolates originating from three published studies. This database uses publicly available next-generation sequence reads from the Sequence Read Archive (SRA). For each isolate, NGS reads were de novo assembled into genome sequences. wgMLST alleles were called using the assembly-based and assembly-free method.
Note that the downloaded database backup file (.bnbk) can be restored via the Restore database... functionality in the BIONUMERICS startup screen.