In this tutorial all steps needed to perform a wgSNP analysis in BioNumerics - with mapping on the external calculation engine - are illustrated using the whole genome demonstration database of Staphylococcus aureus.
wgSNP analysisWhole-genome Single Nucleotide Polymorphism (SNP) analysis using BIONUMERICS. See the wgSNP application page for a complete overview.
Demonstration database containing data for a set of 97 Staphylococcus aureus isolates originating from three published studies. This database uses publicly available next-generation sequence reads from the Sequence Read Archive (SRA). For each isolate, NGS reads were de novo assembled into genome sequences. wgMLST alleles were called using the assembly-based and assembly-free method.
Note that the downloaded database backup file (.bnbk) can be restored via the Restore database... functionality in the BIONUMERICS startup screen.